ctl immunospot s6 elispot plate reader  (Cellular Technology Ltd)

Journal: Vaccines

Article Title: Immunological Analysis of the Hepatitis B Virus “a” Determinant Displayed on Chimeric Virus-Like Particles of Macrobrachium rosenbergii Nodavirus Capsid Protein Produced in Sf 9 Cells

doi: 10.3390/vaccines8020275

Figure Lengend Snippet: Enzyme-linked immunospot (ELISPOT) analysis of activated mice splenocytes.

Article Snippet: Finally, the spots formed by antibody secreting cells were counted with the ELISPOT plate reader (CTL Immunospot S6 Ultimate, CTL, Cleveland, OH, USA).

Techniques: Enzyme-linked Immunospot, Produced

Journal: PLoS ONE

Article Title: Mucosal Adjuvant Activity of IL-2 Presenting Spores of Bacillus subtilis in a Murine Model of Helicobacter pylori Vaccination

doi: 10.1371/journal.pone.0095187

Figure Lengend Snippet: The splenocytes were isolated from naïve mice (open bars), mice orally immunized with 168 spores (dotted open bars), BKH108 (CotC-UreB, vegetative expression of UreB) (bars with horizontal hatching), BKH122 (CotB-linker-IL-2) (bars with vertical hatching) or 1∶1 mixture of BKH108 and BKH122 (dotted closed bars). Cells were treated with purified UreB protein for 72 h and then the IFN-γ cells were enumerated by ELISpot procedure. Error bars represent standard deviation. * p-value <0.05, ** p-value <0.005. I – day 22, II – day 43, III – day 61.

Article Snippet: Splenocytes (2×10 5 /mL) were cultured in presence or absence of UreB antigen for 48 h. The spots were counted using automated ELISpot plate reader (CTL-ImmunoSpot S6 Micro Analyzer, USA).

Techniques: Isolation, Expressing, Purification, Enzyme-linked Immunospot, Standard Deviation